On a small island, a population of ground beetles includes both light-brown and dark-brown individuals. Shell color is inherited: parents pass it on to their offspring. Birds that hunt by sight are the beetles' main predators.
Before a volcano erupted, the island's ground was covered with light, sandy soil, and only about 10% of the beetles were dark. The eruption covered the ground with dark ash that stayed in place for many years. Scientists sampled the beetle population every five generations after the eruption and recorded the percentage of dark-colored beetles.
According to the graph, by how many percentage points did the share of dark-colored beetles increase from generation 0 to generation 20?
percentage points
Type your answer in the box. Fractions like 3/4 are fine.
Hint
Read the value at generation 0 and the value at generation 20, then subtract.
Step-by-step solution
Where the answer comes from: the graph shows 10% dark beetles at generation 0 and 85% at generation 20.
Step by step:
Read the start value: generation 0 is 10%.
Read the end value: generation 20 is 85%.
Subtract: 85 - 10 = 75 percentage points.
Answer: 75 percentage points.
Percentage points vs. percent change: a change from 10% to 85% is an increase of 75 percentage points (a simple difference). The percent change would be \dfrac{75}{10} \times 100 = 750\%, a different quantity. Read which one the question asks for.
Tip: on line graphs, put your finger on the x-axis value, go straight up to the line, then straight across to the y-axis. The labeled data points on this graph make reading easier, but always check the axis titles and units.
Question 2 of 36
Beetles After the Eruption
On a small island, a population of ground beetles includes both light-brown and dark-brown individuals. Shell color is inherited: parents pass it on to their offspring. Birds that hunt by sight are the beetles' main predators.
Before a volcano erupted, the island's ground was covered with light, sandy soil, and only about 10% of the beetles were dark. The eruption covered the ground with dark ash that stayed in place for many years. Scientists sampled the beetle population every five generations after the eruption and recorded the percentage of dark-colored beetles.
Which explanation for the change in the beetle population is best supported by the passage and graph?
Hint
The passage says both colors were already present before the eruption. Ask which beetles birds could see more easily on dark ash.
Step-by-step solution
Where the answer comes from: the passage says the population already "includes both light-brown and dark-brown individuals," that "shell color is inherited," and that birds "hunt by sight." After dark ash covered the ground, the graph shows dark beetles rising from 10% to 85% over 20 generations.
Step by step (the parts of natural selection):
Variation: both colors existed before the eruption.
Inheritance: color is passed from parents to offspring.
Selection: on dark ash, light beetles stand out, so birds eat more of them. Dark beetles are more likely to survive and reproduce.
Time: over many generations, the dark-color trait becomes more common in the population.
Answer: On the dark ash, dark beetles were harder for birds to see, so more of them survived and reproduced, passing dark color to their offspring.
Why the others fail: individual beetles cannot change their inherited shell color because they need to (A). The passage says dark beetles existed before the ash, so the ash did not create them (C). Choice D reverses the predator effect: if birds preferred dark beetles, the dark share would fall.
Tip: natural selection acts on variation that already exists. Populations change over generations; individuals do not evolve during their lifetimes.
Question 3 of 36
Drag and drop
Drag the statements into the boxes to show the steps of natural selection in order. One statement is not used.
Step 1
Step 2
Step 3
Step 4
Tap a tile, then tap a box to place it.
Hint
Natural selection can only work on differences that already exist. What has to come first?
Step-by-step solution
The logic of natural selection (proposed by Charles Darwin and Alfred Russel Wallace in 1858):
Variation: individuals in a population differ in inherited traits. Without variation, there is nothing to select.
Advantage: in a particular environment, some variations help individuals survive (escape predators, find food, resist disease).
Differential reproduction: individuals with helpful traits survive longer and leave more offspring, which inherit those traits.
Change over generations: the helpful trait becomes more common in the population.
The unused statement, "individuals develop new traits because they need them," describes an older, disproven idea associated with Jean-Baptiste Lamarck. Traits an animal gains during its life (like stronger muscles from exercise) are not passed on in its DNA.
Answer: variation → some variations give an advantage → those individuals reproduce more → the trait becomes more common.
Tip: a memory aid is VIST: Variation, Inheritance, Selection, Time.
Question 4 of 36
Whales With Legs
Whales live their whole lives in water, but they are mammals: they breathe air, are warm-blooded and nurse their young. Paleontologists hypothesize that whales evolved from four-legged mammals that lived on land. The table summarizes several fossil animals and modern whales. Ages are approximate.
Animal
Age (millions of years ago)
Description
Pakicetus
50
Four legs; lived on land near water; ear bones shaped like those of modern whales
Ambulocetus
49
Four short legs and large feet; lived both on land and in water
Rodhocetus
47
Short hind legs; probably swam using its tail and legs
Basilosaurus
40
Lived only in water; tiny hind legs that could not support its body
Modern whales
Today
No hind legs; small pelvic and leg bones inside the body, not attached to the spine
According to the table, modern whales have small pelvic and leg bones inside the body that do not support any legs. These bones are best described as
Hint
Think of a body part that was useful to an organism's ancestors but has little or no use today.
Step-by-step solution
Where the answer comes from: the table says modern whales have "No hind legs; small pelvic and leg bones inside the body, not attached to the spine." The fossils show that whale ancestors such as Pakicetus had four working legs.
Step by step:
A vestigial structure is a reduced body part that had a function in an organism's ancestors but has little or no function now.
The whale's hip and leg bones cannot support a leg, but they match the hip bones of four-legged mammals.
Over millions of years, as whale ancestors moved into the water, the hind legs shrank (Rodhocetus: short; Basilosaurus: tiny; modern whales: hidden inside the body).
Answer: vestigial structures, reduced leftovers of body parts that were useful to the whale's ancestors.
Check: the bones are not developing into anything new; the fossils show legs getting smaller over time, not larger.
Tip: vestigial structures are evidence of ancestry: an organism would have no reason to build a useless part unless it inherited the instructions from ancestors that used it.
Question 5 of 36
Beaks After a Drought
On a small island, a population of seed-eating birds called ground finches varies in beak depth (how tall the beak is from top to bottom). Beak depth is largely inherited: parents with deep beaks tend to have offspring with deep beaks. Birds with deeper beaks can crack larger, harder seeds.
One year, a severe drought struck the island. Plants that make small, soft seeds died back, and most of the food left was large, hard seeds. Many finches died during the drought. Researchers measured the beaks of the birds before the drought, of the birds that survived it, and of the offspring hatched the year after.
Group
Average beak depth (mm)
Birds before the drought
9.4
Birds that survived the drought
9.9
Offspring hatched after the drought
9.8
Why did the average beak depth of the finches increase after the drought?
Hint
Compare the birds before the drought with the birds that survived it. Which birds were more likely to make it through, and what did their offspring inherit?
Step-by-step solution
Where the answer comes from: the passage says beak depth "is largely inherited" and that after the drought "most of the food left was large, hard seeds," which deep-beaked birds can crack. The table shows the average beak depth was 9.4 mm before the drought, 9.9 mm in the survivors and 9.8 mm in their offspring.
Step by step:
Variation: finches already differed in beak depth before the drought.
Selection: with mostly hard seeds left, deep-beaked birds could eat and survive; many shallow-beaked birds died. The survivors' average rose to 9.9 mm.
Inheritance: the survivors passed their deep beaks to their young, so the next generation averaged 9.8 mm.
Answer: Finches with deeper beaks were more likely to survive the drought by eating hard seeds, and they passed this trait to their offspring.
Check: the offspring average (9.8 mm) is close to the survivors' average (9.9 mm), which shows the trait was inherited.
Tip: a correct natural selection answer always has three parts: existing variation, unequal survival or reproduction, and inheritance.
Question 6 of 36
Fill inAntibiotic Resistance in a Hospital
Staphylococcus aureus is a common bacterium that can cause skin and blood infections. In 2000, doctors at a large hospital began using a new antibiotic widely. Each year, the hospital lab tested samples of the bacterium taken from patients to find what percent could survive the antibiotic (were resistant).
Even before 2000, a few bacteria in the population carried a gene variant that let them survive the antibiotic. Resistance is inherited: when a resistant bacterium divides, both new cells are resistant. In 2014, the hospital started a program that cut the use of the antibiotic roughly in half.
Use the graph. From 2000 to 2012 the share of resistant samples went up. What was the size of this increase?
percentage points
Type your answer in the box. Fractions like 3/4 are fine.
Hint
Read the value for 2000 and the value for 2012, then subtract.
Step-by-step solution
Where the answer comes from: the graph shows 5% resistant samples in 2000 and 38% in 2012.
Step by step:
Find each value on the line: 2000 → 5; 2012 → 38.
Subtract the earlier value from the later one: 38 - 5 = 33.
Answer: 33 percentage points.
Check: count the steps along the way: 5 \to 10 \to 22 \to 38 means increases of 5 + 12 + 16 = 33 ✓
Tip: a change from one percent to another is measured in percentage points, found by subtracting. Do not divide: 38 \div 5 would give a ratio (7.6 times), which answers a different question.
Question 7 of 36
A Vertebrate Cladogram
A cladogram is a branching diagram that shows how groups of organisms are related through common ancestors. Each branch point (node) represents the most recent common ancestor of all the organisms on the branches above it.
The marks on the main line show shared derived traits. A trait first appears at the point where it is marked, and it is shared by every organism whose branch splits off after (above) that point. Organisms that share a more recent common ancestor (a node closer to the top) are more closely related.
According to the cladogram, which trait is shared by the frog, lizard and mouse but not by the shark?
Hint
A trait is shared by every organism whose branch splits off after the trait's mark. Which mark comes right after the shark's branch?
Step-by-step solution
Where the answer comes from: the passage says a trait "is shared by every organism whose branch splits off after (above) that point." On the cladogram, the order along the main line is: vertebral column, Lamprey branch, jaws, Shark branch, four limbs, Frog branch, amniotic egg, Lizard branch, hair, Mouse.
Step by step:
Find the shark's branch point. The next mark above it is four limbs.
Four limbs is shared by every animal above that mark: frog, lizard and mouse. The shark branched off before it, so the shark lacks it.
Check the others:
Jaws appears before the shark branch, so the shark has jaws too.
Amniotic egg appears after the frog branch, so the frog lacks it.
Vertebral column is shared by all five animals.
Answer: four limbs.
Tip: on a cladogram, read the traits like a timeline along the main line. Everything above a trait mark has that trait; everything that branched off below it does not.
Question 8 of 36
A Vertebrate Cladogram
A cladogram is a branching diagram that shows how groups of organisms are related through common ancestors. Each branch point (node) represents the most recent common ancestor of all the organisms on the branches above it.
The marks on the main line show shared derived traits. A trait first appears at the point where it is marked, and it is shared by every organism whose branch splits off after (above) that point. Organisms that share a more recent common ancestor (a node closer to the top) are more closely related.
According to the cladogram, which animal is most closely related to the lizard?
Hint
Relatedness depends on which pair shares the most recent common ancestor (the highest branch point), not on whose name is printed next to the lizard's.
Step-by-step solution
Where the answer comes from: the passage says "organisms that share a more recent common ancestor (a node closer to the top) are more closely related." On the cladogram, the lizard's branch splits off at the highest node, and the line continues only to the mouse.
Step by step:
Find the node where the lizard's branch splits off. It is the top node.
The only other animal above that node is the mouse, so the lizard and the mouse share the most recent common ancestor.
The frog split off earlier, at a lower node. The frog's common ancestor with the lizard is older, and it is also the ancestor of the mouse.
Supporting evidence: the lizard and the mouse share the amniotic egg, a trait the frog lacks. (Mammals' amniotic membranes surround the embryo even though most mammals do not lay eggs.)
Answer: mouse.
Why "frog" is tempting: the frog's name is printed right next to the lizard's, and both are often thought of as "cold-blooded." But a cladogram groups organisms by shared ancestry and derived traits, not by position on the page or by how similar they look.
Tip: to compare relatedness, trace both branches down to where they meet. The higher the meeting point, the closer the relationship.
Question 9 of 36
Fossil Horses
Fossils of horses and their ancestors have been found in rock layers in North America. Fossils from deeper rock layers are older. The table summarizes several fossil groups. Ages and sizes are approximate.
Fossil group
Age (millions of years ago)
Shoulder height (meters)
Toes on each front foot
Teeth
Eohippus
55
0.4
4
Low-crowned, suited to soft leaves
Mesohippus
35
0.6
3
Low-crowned
Merychippus
15
1.0
3 (weight on the large middle toe)
High-crowned, suited to grinding tough grass
Pliohippus
10
1.2
1
High-crowned
Equus (modern horse)
4 to present
1.6
1
High-crowned
A paleontologist proposes this hypothesis: High-crowned teeth became common in horses because their ancestors began eating tough grasses as grasslands spread across North America.
Which trend in horse evolution is best supported by the table?
Hint
Read the table from the oldest fossil (55 million years ago) to the present. How do height and toe number change?
Step-by-step solution
Where the answer comes from: the table lists the fossil groups from oldest to youngest: Eohippus (55 million years ago), Mesohippus (35), Merychippus (15), Pliohippus (10), Equus (4 to present).
Step by step:
Shoulder height: 0.4 → 0.6 → 1.0 → 1.2 → 1.6 m, so horses got taller.
Toes on each front foot: 4 → 3 → 3 → 1 → 1, so the number of toes decreased.
Teeth: low-crowned in the two oldest groups, then high-crowned from Merychippus on, so teeth changed from low-crowned to high-crowned, not the reverse.
Answer: Over time, horses became larger and the number of toes on each front foot decreased.
Why the others fail: B reverses the toe trend. C reverses the size trend. D reverses the tooth trend; the oldest groups had low-crowned teeth.
Tip: when reading a table of change over time, first confirm which direction is "older." Many wrong answers on the GED simply reverse a trend.
Question 10 of 36
Fossil Horses
Fossils of horses and their ancestors have been found in rock layers in North America. Fossils from deeper rock layers are older. The table summarizes several fossil groups. Ages and sizes are approximate.
Fossil group
Age (millions of years ago)
Shoulder height (meters)
Toes on each front foot
Teeth
Eohippus
55
0.4
4
Low-crowned, suited to soft leaves
Mesohippus
35
0.6
3
Low-crowned
Merychippus
15
1.0
3 (weight on the large middle toe)
High-crowned, suited to grinding tough grass
Pliohippus
10
1.2
1
High-crowned
Equus (modern horse)
4 to present
1.6
1
High-crowned
A paleontologist proposes this hypothesis: High-crowned teeth became common in horses because their ancestors began eating tough grasses as grasslands spread across North America.
Which additional finding would best support the paleontologist's hypothesis?
Hint
The hypothesis links two events: grasslands spreading and high-crowned teeth appearing. Which finding connects them in time?
Step-by-step solution
Where the answer comes from: the hypothesis says high-crowned teeth became common "because their ancestors began eating tough grasses as grasslands spread across North America." The table shows high-crowned teeth first appearing in Merychippus, about 15 million years ago.
Step by step:
To support a cause-and-effect claim, show that the cause (grasslands spreading) happened at the right time, just before or alongside the effect (high-crowned teeth).
Fossil grass pollen and plant remains showing that grasslands became widespread at about the time high-crowned teeth appear would directly link the two.
Check the others:
Modern horses preferring grass says nothing about when or why the teeth changed millions of years ago.
More Eohippus fossils with low-crowned teeth repeat what we already know about one early group.
Measuring modern horse heights is unrelated to teeth or diet.
Answer: Fossil pollen and plant remains showing that grasslands became widespread in North America around the time high-crowned teeth appear.
Tip: the best supporting evidence tests the specific link in the hypothesis. Evidence that is true but does not connect the cause to the effect is "true but irrelevant."
Question 11 of 36
Comparing a Protein
Cytochrome c is a protein that helps release energy in the mitochondria of animals, plants and fungi. In humans it is a chain of 104 amino acids. Because the protein is built from instructions in DNA, scientists can compare its amino acid sequence in different species. Fewer differences suggest that two species share a more recent common ancestor.
The table shows the approximate number of amino acids in each species' cytochrome c that differ from the human sequence.
Species
Amino acid differences from humans
Chimpanzee
0
Rhesus monkey
1
Horse
12
Chicken
13
Tuna
21
Baker's yeast
44
Based on the data, which list orders the species from most closely to least closely related to humans?
Hint
Fewer amino acid differences mean a more recent common ancestor. Sort the species by their numbers, from smallest to largest.
Step-by-step solution
Where the answer comes from: the passage says "fewer differences suggest that two species share a more recent common ancestor." The table gives: chimpanzee 0, rhesus monkey 1, horse 12, chicken 13, tuna 21, baker's yeast 44.
Step by step:
Sort the differences from smallest to largest: 0, 1, 12, 13, 21, 44.
Match the species: chimpanzee, rhesus monkey, horse, chicken, tuna, yeast.
Smallest difference = most closely related, so this order runs from most to least closely related.
Why it makes sense: this order matches other lines of evidence. Chimpanzees and monkeys are primates like us; horses are mammals; chickens are vertebrates with amniotic eggs; tuna are vertebrates without them; yeast is a fungus. Independent evidence from anatomy, fossils and molecules pointing to the same branching pattern is strong support for common ancestry.
Tip: in "differences" tables, small numbers mean close relatives. In "similarity" tables (percent of DNA shared), large numbers mean close relatives. Check which kind of table you have.
Question 12 of 36
Drop-downLizards on Two Islands
Scientists are studying two populations of a small lizard that live on neighboring islands, Island A and Island B. Thousands of years ago, the islands were joined by a strip of land, and the lizards formed a single population. Then the sea level rose and covered the land bridge. The lizards cannot swim between the islands, so the two populations no longer mix.
Over many generations, the populations have changed in different ways. Island A is rocky, with few plants, and its lizards now have longer legs on average, which help them run quickly across open rock. Island B is covered with thin bushes, and its lizards have shorter legs on average, which help them grip narrow branches. Genetic tests show that the two populations descended from the same ancestral population.
The scientists want to know whether the two populations have now become separate species.
Select the words that correctly complete the scientists' summary.
The rising sea level that covered the land bridge is an example of . The difference in average leg length between the two populations most likely developed through . If lizards from the two islands can no longer produce fertile offspring together, the populations will be considered .
Choose an answer from each menu.
Hint
Think about the three stages of speciation: a barrier separates a population, the separated groups face different conditions, and eventually they can no longer interbreed.
Step-by-step solution
Where the answer comes from: the passage says the sea "covered the land bridge," so the lizards "no longer mix." Island A's longer legs "help them run quickly across open rock," and Island B's shorter legs "help them grip narrow branches."
Step by step:
A physical barrier (water) splitting one population into two is geographic isolation.
Each island favored a different leg length, a trait that helps survival in that habitat. Individuals with the helpful leg length survived and reproduced more, so leg length changed through natural selection.
The usual biological definition of a species is a group whose members can interbreed and produce fertile offspring. If the island populations cannot, they are separate species.
Answer: geographic isolation; natural selection; separate species.
Why the wrong items fail: "mutation caused by seawater" invents a cause; nothing in the passage says the water changed DNA. "Individual lizards stretching their legs" is the disproven idea that acquired traits are inherited. "Still one species" contradicts the definition of a species.
Tip: speciation usually follows the pattern isolation → different selection pressures → reproductive isolation.
Question 13 of 36
Select allA Plant Cladogram
The cladogram shows how five kinds of plants and algae are related. Each branch point (node) represents the most recent common ancestor of all the organisms on the branches above it.
A mark on the main line shows where a trait first appeared. Every organism whose branch splits off above that mark has the trait.
According to the cladogram, which organisms have vascular tissue? Select all that apply.
Select all that apply.
Hint
Find the vascular tissue mark on the main line. Which branches split off above it?
Step-by-step solution
Where the answer comes from: the passage says "Every organism whose branch splits off above that mark has the trait." On the cladogram, the vascular tissue mark sits on the main line after the moss branch and before the fern branch.
Step by step:
Locate the mark: between the moss node and the fern node.
Branches that split off above it: fern, pine and sunflower. All three have vascular tissue.
Branches that split off below it: green alga and moss. They do not have vascular tissue (mosses move water slowly from cell to cell, which is one reason they stay small).
Answer: fern, pine and sunflower.
Check: later traits are nested inside earlier ones: everything with seeds (pine, sunflower) also has vascular tissue, because the seed mark comes after the vascular mark.
Tip: on a cladogram, a trait is shared by everything past its mark, not just the next branch.
Question 14 of 36
Same Bones, Different Jobs
The front limbs of many vertebrates do very different jobs. A human arm grasps tools, a cat's front leg walks and runs, a whale's flipper steers through water, and a bat's wing flies. Yet all four limbs contain the same set of bones in the same order: one upper-arm bone, two forearm bones, a group of wrist bones, and finger bones. Structures like these, which share the same basic plan because they were inherited from a common ancestor, are called homologous structures.
Other structures do the same job but are built in very different ways. A bird's wing is supported by bones and covered with feathers, while a butterfly's wing is a thin sheet with no bones at all. Such structures are called analogous structures. They evolved separately in groups that do not share a winged ancestor.
Based on the passage, which is the best explanation for why a bat's wing and a whale's flipper contain the same set of bones?
Hint
The two limbs do completely different jobs. So what, besides the job, could explain why they are built the same way?
Step-by-step solution
Where the answer comes from: paragraph 1 says the limbs "share the same basic plan because they were inherited from a common ancestor" and calls them homologous structures.
Step by step:
Flying and swimming are very different jobs, so the shared bones cannot be explained by a shared job.
The best explanation is that bats and whales both descended from an early mammal whose front limb had this set of bones.
Over time, natural selection reshaped the same bones in different ways: long thin finger bones in the bat's wing, short flat bones in the whale's flipper.
Answer: Bats and whales inherited the same basic limb plan from a common ancestor.
Check: the passage contrasts this with analogous structures (bird and butterfly wings), which do the same job with different structures. Bat wing and whale flipper are the opposite: different jobs, same structure.
Tip: same bones + different jobs = homologous = common ancestry. Same job + different structure = analogous = evolved separately.
Question 15 of 36
Drag and dropBeaks After a Drought
On a small island, a population of seed-eating birds called ground finches varies in beak depth (how tall the beak is from top to bottom). Beak depth is largely inherited: parents with deep beaks tend to have offspring with deep beaks. Birds with deeper beaks can crack larger, harder seeds.
One year, a severe drought struck the island. Plants that make small, soft seeds died back, and most of the food left was large, hard seeds. Many finches died during the drought. Researchers measured the beaks of the birds before the drought, of the birds that survived it, and of the offspring hatched the year after.
Group
Average beak depth (mm)
Birds before the drought
9.4
Birds that survived the drought
9.9
Offspring hatched after the drought
9.8
Put these events from the finch study in the order in which they happened as natural selection changed the population. Drag each statement into a box; one statement does not belong.
Step 1
Step 2
Step 3
Step 4
Tap a tile, then tap a box to place it.
Hint
Selection can act only on differences that already exist. Then something in the environment must make some of those differences matter.
Step-by-step solution
Where the answer comes from: the passage says the finch population "varies in beak depth," that beak depth "is largely inherited," and that after the drought "most of the food left was large, hard seeds." The table shows the average rising from 9.4 mm to 9.8 mm in the next generation.
Step by step:
Variation: finches differ in inherited beak depth.
Environmental pressure: the drought leaves mostly large, hard seeds.
Unequal survival and reproduction: finches with deeper beaks survive and reproduce more.
Change in the population: the next generation has a deeper average beak.
Not used: "Each finch stretches its beak to reach the hard seeds" describes individuals changing because of need, an idea that is not how inherited traits change.
Answer: variation → drought leaves hard seeds → deep-beaked finches survive and reproduce more → deeper average beak in the next generation.
Check: the population changed, but no single bird's beak changed. Only the mix of birds changed.
Tip: natural selection acts on individuals, but only populations evolve.
Question 16 of 36
From Wild Mustard to the Supermarket
Cabbage, broccoli, cauliflower, kale, kohlrabi and Brussels sprouts look very different, yet they all belong to the same species, Brassica oleracea. All of them were developed from a wild mustard plant that grows along the coasts of western Europe.
Over hundreds of years, farmers saved seeds only from the plants that had the traits they wanted and planted those seeds the next year. This process is called artificial selection, or selective breeding.
Vegetable
Trait farmers selected for
Kale
Large leaves
Cabbage
A large bud at the tip of the stem
Broccoli and cauliflower
Thick clusters of flower buds
Kohlrabi
A swollen stem
Brussels sprouts
Many buds along the side of the stem
How does the process described in the passage differ from natural selection?
Hint
In the passage, who decided which mustard plants would produce the next generation? In nature, what decides?
Step-by-step solution
Where the answer comes from: paragraph 2 says "farmers saved seeds only from the plants that had the traits they wanted" and calls this artificial selection.
Step by step:
Artificial selection: people choose which individuals reproduce, based on traits people want (big leaves, big flower clusters).
Natural selection: no one chooses. The environment (food, predators, climate) determines which individuals survive and reproduce more.
What they share: both need inherited variation, and both make chosen traits more common over generations. The farmers did not create new traits; they picked from variation already present in the wild mustard.
Answer: In artificial selection, people choose which individuals reproduce; in natural selection, the environment determines which individuals survive and reproduce more.
Check: the passage's six vegetables all came from one species in only hundreds of years, which shows how strong selection can be when people pick the parents every generation.
Tip: "artificial" = human-made choice; "natural" = nature's "choice" through survival and reproduction.
Question 17 of 36
Fossils in a Cliff
A geologist studied a cliff made of five layers of sedimentary rock. The layers have not been folded or overturned, so each layer is younger than the layers below it. A thin bed of volcanic ash lies between Layer 3 and Layer 4. Using radioactive dating, the geologist found that the ash is about 250 million years old.
Layer
Fossils found
5 (top)
Mammal teeth and bones; pollen from flowering plants
4
Reptile bones; fern spores; pollen from cone-bearing trees
Based on the passage, which statement about the amphibian bones in Layer 3 is best supported?
Hint
Where is Layer 3 compared with the ash bed? Lower layers are older.
Step-by-step solution
Where the answer comes from: the passage says "each layer is younger than the layers below it," that the ash bed "lies between Layer 3 and Layer 4," and that the ash is "about 250 million years old."
Step by step:
Layer 3 lies below the ash bed, so it formed before the ash fell.
Therefore Layer 3, and the amphibian bones in it, are older than about 250 million years.
Check the others: Layer 3 sits above Layer 2, so the amphibians are younger than the jawed fish; Layer 4 (reptiles) is above the ash, so it is younger than Layer 3, not the same age.
Answer: The amphibian bones are more than about 250 million years old.
Check: the rule only works because the passage says the layers "have not been folded or overturned." In a folded cliff, the order could be upside down.
Tip: a dated layer (like volcanic ash) works as a bookmark: everything below it is older, everything above it is younger.
Question 18 of 36
Antibiotic Resistance in a Hospital
Staphylococcus aureus is a common bacterium that can cause skin and blood infections. In 2000, doctors at a large hospital began using a new antibiotic widely. Each year, the hospital lab tested samples of the bacterium taken from patients to find what percent could survive the antibiotic (were resistant).
Even before 2000, a few bacteria in the population carried a gene variant that let them survive the antibiotic. Resistance is inherited: when a resistant bacterium divides, both new cells are resistant. In 2014, the hospital started a program that cut the use of the antibiotic roughly in half.
Which statement best explains the rise in resistant samples from 2000 to 2012?
Hint
The passage says something about the bacteria before 2000. Did the antibiotic create resistance, or did it change which bacteria survived?
Step-by-step solution
Where the answer comes from: the passage says "Even before 2000, a few bacteria in the population carried a gene variant that let them survive the antibiotic" and "Resistance is inherited." The graph shows resistance rising from 5% in 2000 to 38% in 2012.
Step by step:
Variation: a few resistant bacteria already existed.
Selection: each time the antibiotic was used, it killed most non-resistant bacteria, but the resistant ones survived.
Inheritance and reproduction: the survivors divided, and their offspring were resistant too.
Result: year after year, resistant bacteria made up a larger share of the population.
Answer: The antibiotic killed non-resistant bacteria, while resistant bacteria survived and passed resistance on as they reproduced.
Check: this is natural selection happening fast, because bacteria can produce many generations in a single day.
Tip: the antibiotic is the selecting force, not the cause of the resistance gene. The variation must already be there.
Question 19 of 36
Beaks After a Drought
On a small island, a population of seed-eating birds called ground finches varies in beak depth (how tall the beak is from top to bottom). Beak depth is largely inherited: parents with deep beaks tend to have offspring with deep beaks. Birds with deeper beaks can crack larger, harder seeds.
One year, a severe drought struck the island. Plants that make small, soft seeds died back, and most of the food left was large, hard seeds. Many finches died during the drought. Researchers measured the beaks of the birds before the drought, of the birds that survived it, and of the offspring hatched the year after.
Group
Average beak depth (mm)
Birds before the drought
9.4
Birds that survived the drought
9.9
Offspring hatched after the drought
9.8
A student writes: "During the drought, each finch grew a deeper beak because it needed to crack the hard seeds." Which statement best explains the error in the student's reasoning?
Hint
Look at the table: did the change show up because individual beaks grew, or because the mix of birds changed?
Step-by-step solution
Where the answer comes from: the passage says "Many finches died during the drought" and the table shows that the survivors averaged 9.9 mm, compared with 9.4 mm before. Beak depth "is largely inherited."
Step by step:
The student describes individual birds changing their bodies because of a need. That is not how inherited traits change.
What actually happened: birds that already had deeper beaks were more likely to survive; many shallow-beaked birds died.
The survivors' average was higher because of who survived, not because any bird's beak grew. Their offspring inherited deep beaks.
Answer: Individual finches do not change their inherited traits because of need; the average changed because finches that already had deeper beaks survived and reproduced.
Check: even if a bird's beak did wear or grow during its life, such changes are not stored in its DNA, so they would not be passed to its offspring.
Tip: watch for the words needed to, in order to, or tried to in an explanation of evolution. They usually signal the need-based (Lamarckian) error.
Question 20 of 36
Antibiotic Resistance in a Hospital
Staphylococcus aureus is a common bacterium that can cause skin and blood infections. In 2000, doctors at a large hospital began using a new antibiotic widely. Each year, the hospital lab tested samples of the bacterium taken from patients to find what percent could survive the antibiotic (were resistant).
Even before 2000, a few bacteria in the population carried a gene variant that let them survive the antibiotic. Resistance is inherited: when a resistant bacterium divides, both new cells are resistant. In 2014, the hospital started a program that cut the use of the antibiotic roughly in half.
During which 4-year period did the percent of resistant samples increase the most?
Hint
Find the change for each period by subtracting the starting value from the ending value. The steepest part of the line has the biggest change.
Step-by-step solution
Where the answer comes from: the graph gives these values: 2000: 5; 2004: 10; 2008: 22; 2012: 38; 2016: 46; 2020: 48.
Step by step: subtract for each 4-year period.
2000–2004: 10 - 5 = 5 points
2004–2008: 22 - 10 = 12 points
2008–2012: 38 - 22 = 16 points
2012–2016: 46 - 38 = 8 points
2016–2020: 48 - 46 = 2 points
Answer: 2008 to 2012.
Check: on the graph, the segment from 2008 to 2012 is the steepest part of the line, which matches the largest change.
Tip: "increased the most" asks about the change during a period, not the highest value. The highest value (48) is in 2020, but the line was almost flat by then.
Question 21 of 36
Comparing Primate DNA
Scientists compared long stretches of DNA from humans and several other primates. The table shows the approximate percent of DNA bases that differ between humans and each species.
After two groups split from a common ancestor, small changes build up in each group's DNA slowly and fairly steadily. So fewer differences suggest a more recent common ancestor.
Species
DNA difference from humans (%)
Chimpanzee
1.2
Gorilla
1.6
Orangutan
3.1
Rhesus macaque (a monkey)
7.0
What do the DNA comparisons in the table best support about how humans are related to the other primates?
Hint
Fewer DNA differences mean a more recent common ancestor. Check that each choice uses only comparisons with humans, since that is all the table gives.
Step-by-step solution
Where the answer comes from: the passage says "fewer differences suggest a more recent common ancestor." The table shows humans differ from chimpanzees by 1.2% and from orangutans by 3.1%.
Step by step:
1.2% (chimpanzee) is less than 3.1% (orangutan).
So humans share a more recent common ancestor with chimpanzees than with orangutans.
Check the others: the table does not say humans came from chimpanzees; gorillas (1.6%) differ more than chimps (1.2%); and the table compares each species only with humans, so it cannot tell us how orangutans and macaques compare with each other.
Answer: Humans share a more recent common ancestor with chimpanzees than with orangutans.
Check: the full order from most to least closely related to humans is chimpanzee (1.2) → gorilla (1.6) → orangutan (3.1) → macaque (7.0).
Tip: a table of differences from one species supports conclusions about that species only. Comparing two other species needs data for that pair.
Question 22 of 36
Whales With Legs
Whales live their whole lives in water, but they are mammals: they breathe air, are warm-blooded and nurse their young. Paleontologists hypothesize that whales evolved from four-legged mammals that lived on land. The table summarizes several fossil animals and modern whales. Ages are approximate.
Animal
Age (millions of years ago)
Description
Pakicetus
50
Four legs; lived on land near water; ear bones shaped like those of modern whales
Ambulocetus
49
Four short legs and large feet; lived both on land and in water
Rodhocetus
47
Short hind legs; probably swam using its tail and legs
Basilosaurus
40
Lived only in water; tiny hind legs that could not support its body
Modern whales
Today
No hind legs; small pelvic and leg bones inside the body, not attached to the spine
Which additional finding would best support the hypothesis that whales evolved from four-legged land mammals?
Hint
Look for a finding that connects modern whales to legs or to land mammals. Facts about how whales live today do not test where they came from.
Step-by-step solution
Where the answer comes from: the passage states the hypothesis: "whales evolved from four-legged mammals that lived on land." The fossils show legs shrinking over time, and modern whales keep "small pelvic and leg bones inside the body."
Step by step:
A supporting finding should show a trace of four-legged ancestry in whales.
Hind-limb buds in whale embryos are exactly that: the instructions for building legs are still in whale DNA, switched on briefly and then turned off. (This has in fact been observed in dolphin embryos.)
The other choices are true or plausible facts about whales or sharks, but they say nothing about whale ancestry. A shark's streamlined shape is an analogous trait shared by unrelated swimmers.
Answer: Early in development, whale embryos grow small hind-limb buds that later disappear.
Check: this finding fits the fossil pattern too: the ancestors had legs, the descendants lost them, and the embryos still show a brief trace.
Tip: to support a hypothesis about ancestry, look for evidence that links the organism to its supposed ancestors (shared bones, embryos, DNA). A fact about the present-day lifestyle is usually irrelevant.
Question 23 of 36
Comparing a Protein
Cytochrome c is a protein that helps release energy in the mitochondria of animals, plants and fungi. In humans it is a chain of 104 amino acids. Because the protein is built from instructions in DNA, scientists can compare its amino acid sequence in different species. Fewer differences suggest that two species share a more recent common ancestor.
The table shows the approximate number of amino acids in each species' cytochrome c that differ from the human sequence.
Species
Amino acid differences from humans
Chimpanzee
0
Rhesus monkey
1
Horse
12
Chicken
13
Tuna
21
Baker's yeast
44
A student looks at the table and concludes: "Tuna evolved into chickens, and chickens then evolved into horses, because the number of differences from humans gets smaller from tuna to chicken to horse." Which statement best evaluates the student's conclusion?
Hint
The table compares each living species with humans. Does it show one living species turning into another, or does it show how recently species share ancestors?
Step-by-step solution
Where the answer comes from: the passage says the table shows differences "from the human sequence," and that "fewer differences suggest that two species share a more recent common ancestor."
Step by step:
Tuna, chickens and horses are all living today. A living species cannot be the ancestor of another living species; each has had the same amount of time to evolve since their lineages split.
The data measure similarity to humans. They suggest that humans share a more recent common ancestor with horses than with chickens, and with chickens than with tuna.
That supports a pattern of branching from common ancestors, like a cladogram, not a ladder in which one modern species turns into the next.
Answer: The conclusion is not supported. The data suggest how recently each species shares a common ancestor with humans; they do not show that one living species evolved into another.
Why the others fail: A accepts the faulty "ladder" reasoning. B is wrong because the data are relevant to relatedness; they just do not support this conclusion. D treats the 1-amino-acid gap between horse (12) and chicken (13) as proof of descent, but closeness to humans says nothing about whether horses came from chickens.
Tip: common ancestry means branching. Humans did not evolve from chimpanzees; both evolved from a shared ancestor. Watch for GED choices that describe evolution as a straight line between living species.
Question 24 of 36
Hot spotA Vertebrate Cladogram
A cladogram is a branching diagram that shows how groups of organisms are related through common ancestors. Each branch point (node) represents the most recent common ancestor of all the organisms on the branches above it.
The marks on the main line show shared derived traits. A trait first appears at the point where it is marked, and it is shared by every organism whose branch splits off after (above) that point. Organisms that share a more recent common ancestor (a node closer to the top) are more closely related.
Click on the branch point (node) that represents the most recent common ancestor of the shark and the mouse.
Click on the figure to mark your answer (or focus it and use the arrow keys).
Hint
Trace the shark's branch down to where it meets the main line. The mouse's lineage passes through that same point.
Step-by-step solution
Where the answer comes from: the passage says "each branch point (node) represents the most recent common ancestor of all the organisms on the branches above it." On the cladogram, the shark's branch joins the main line at the second node from the bottom. The main line continues from there up to the mouse.
Step by step:
Follow the shark's branch down and to the right until it meets the main line. That node is where the shark's lineage and the lineage leading to the frog, lizard and mouse split.
Every animal above that node (shark, frog, lizard, mouse) descends from it, so it is the most recent ancestor that the shark and the mouse share.
Check the others:
The lowest node (where the lamprey branches) is an older ancestor shared by all five animals, including the lamprey. It is a common ancestor of the shark and mouse, but not the most recent one.
The third node (frog branch) and fourth node (lizard branch) are ancestors of the mouse but not of the shark, which had already split off.
Answer: the second node from the bottom, where the shark's branch joins the main line.
Tip: "most recent common ancestor" of two organisms = the node where their two paths first meet when you trace both down the tree.
Question 25 of 36
Fossils in a Cliff
A geologist studied a cliff made of five layers of sedimentary rock. The layers have not been folded or overturned, so each layer is younger than the layers below it. A thin bed of volcanic ash lies between Layer 3 and Layer 4. Using radioactive dating, the geologist found that the ash is about 250 million years old.
Layer
Fossils found
5 (top)
Mammal teeth and bones; pollen from flowering plants
4
Reptile bones; fern spores; pollen from cone-bearing trees
Which conclusion is best supported by the order of the fossils in the cliff?
Hint
Lower layers are older. Compare where each group of animals first appears, and reject choices that claim more than the layers show.
Step-by-step solution
Where the answer comes from: the table lists, from bottom to top: Layer 1 trilobites and shelled sea animals; Layer 2 jawed fish; Layer 3 amphibians; Layer 4 reptiles; Layer 5 mammals. The passage says "each layer is younger than the layers below it."
Step by step:
Jawed fish first appear in Layer 2.
Amphibians appear in Layer 3, reptiles in Layer 4, mammals in Layer 5. All are higher, so all are younger.
So fish fossils are found in older layers than the first amphibian, reptile and mammal fossils.
Check the others: trilobites and mammals are in different layers (1 and 5), so they did not live at the same time here; the ash only dates the rock, it does not cause anything; and fossils in neighboring layers do not prove one group turned directly into the next.
Answer: Fossils of fish appear in older layers than the first fossils of amphibians, reptiles and mammals.
Check: this matches the wider fossil record, in which fish appear long before the first four-legged land animals.
Tip: the safest conclusion from a rock column is about order (what came before what). Claims about causes or direct ancestors need more evidence.
Question 26 of 36
Hot spotA Plant Cladogram
The cladogram shows how five kinds of plants and algae are related. Each branch point (node) represents the most recent common ancestor of all the organisms on the branches above it.
A mark on the main line shows where a trait first appeared. Every organism whose branch splits off above that mark has the trait.
Click on the mark that shows where the trait shared by the pine and the sunflower, but not by the fern, first appeared.
Click on the figure to mark your answer (or focus it and use the arrow keys).
Hint
Find the fern branch. The trait you need is marked on the main line just above where the fern splits off, and below where the pine splits off.
Step-by-step solution
Where the answer comes from: the passage says a trait is shared by "every organism whose branch splits off above that mark." On the cladogram, the Seeds mark lies on the main line after the fern branch and before the pine branch.
Step by step:
The trait must sit below the pine's branch point (so the pine and the sunflower have it) but above the fern's branch point (so the fern lacks it).
Only one mark sits between the fern node and the pine node: Seeds.
Check the others: Vascular tissue comes before the fern branch, so ferns have it too; Flowers comes after the pine branch, so the pine lacks it; Embryo protected by parent is shared by every plant from the moss on.
Answer: click the Seeds mark.
Check: ferns reproduce with spores, not seeds, while pines (in cones) and sunflowers (in flowers) make seeds, which matches the diagram.
Tip: to find the trait that separates two groups, look between the two branch points on the main line.
Question 27 of 36
A Plant Cladogram
The cladogram shows how five kinds of plants and algae are related. Each branch point (node) represents the most recent common ancestor of all the organisms on the branches above it.
A mark on the main line shows where a trait first appeared. Every organism whose branch splits off above that mark has the trait.
According to the cladogram, which statement best explains why the pine is more closely related to the sunflower than to the moss?
Hint
Trace the pine and the sunflower down to the node where their paths meet. Then do the same for the pine and the moss. Which node is more recent (higher)?
Step-by-step solution
Where the answer comes from: the passage says "Each branch point (node) represents the most recent common ancestor of all the organisms on the branches above it."
Step by step:
Pine and sunflower: their paths meet at the highest node, where the pine splits off.
Pine and moss: their paths meet much lower, at the node where the moss splits off.
A higher node is a more recent common ancestor. Pine and sunflower share a more recent ancestor, so they are more closely related.
The pine and the sunflower also share more marked traits (embryo protection, vascular tissue, seeds) than the pine and the moss (only embryo protection).
Answer: The pine and the sunflower share a more recent common ancestor than the pine and the moss do.
Check: the pine does not have flowers, because the Flowers mark lies above the pine branch.
Tip: relatedness on a cladogram is about how recently two lineages split, not about which organism "came from" which.
Question 28 of 36
Drag and dropSame Bones, Different Jobs
The front limbs of many vertebrates do very different jobs. A human arm grasps tools, a cat's front leg walks and runs, a whale's flipper steers through water, and a bat's wing flies. Yet all four limbs contain the same set of bones in the same order: one upper-arm bone, two forearm bones, a group of wrist bones, and finger bones. Structures like these, which share the same basic plan because they were inherited from a common ancestor, are called homologous structures.
Other structures do the same job but are built in very different ways. A bird's wing is supported by bones and covered with feathers, while a butterfly's wing is a thin sheet with no bones at all. Such structures are called analogous structures. They evolved separately in groups that do not share a winged ancestor.
Drag one example into each box. One example is not used.
Homologous structures
Analogous structures
Vestigial structure
Tap a tile, then tap a box to place it.
Hint
Homologous: same bones, different jobs. Analogous: same job, different build. Vestigial: a reduced part with little or no use today.
Step-by-step solution
Where the answer comes from: the passage defines homologous structures as limbs that "share the same basic plan because they were inherited from a common ancestor," and analogous structures as structures that "do the same job but are built in very different ways."
Step by step:
Homologous: a bat's wing and a cat's front leg contain the same set of bones but do different jobs (flying, walking).
Analogous: a butterfly's wing and a bird's wing both fly, but one has bones and feathers and the other is a thin sheet with no bones. They evolved separately.
Vestigial: the small pelvic bones inside a whale are reduced leftovers of the hips of four-legged ancestors and support no legs today.
Not used: two kittens with different fur colors show variation within a species, not anatomical evidence comparing different groups.
Answer: Homologous = bat wing and cat front leg; Analogous = butterfly wing and bird wing; Vestigial = whale pelvic bones.
Check: only homologous and vestigial structures point to common ancestry; analogous structures show that similar environments can shape unrelated groups in similar ways.
Tip: do not judge by the job alone. Ask "Is it built the same way inside?" before deciding.
Question 29 of 36
Beaks After a Drought
On a small island, a population of seed-eating birds called ground finches varies in beak depth (how tall the beak is from top to bottom). Beak depth is largely inherited: parents with deep beaks tend to have offspring with deep beaks. Birds with deeper beaks can crack larger, harder seeds.
One year, a severe drought struck the island. Plants that make small, soft seeds died back, and most of the food left was large, hard seeds. Many finches died during the drought. Researchers measured the beaks of the birds before the drought, of the birds that survived it, and of the offspring hatched the year after.
Group
Average beak depth (mm)
Birds before the drought
9.4
Birds that survived the drought
9.9
Offspring hatched after the drought
9.8
Suppose several rainy years follow the drought. Plants with small, soft seeds become plentiful again, and large, hard seeds become rare. Finches with shallower beaks can pick up and eat small seeds faster than finches with deep beaks. What would most likely happen to the finch population over the next several generations?
Hint
Natural selection favors whatever trait works best in the current environment. Which beaks would help most now?
Step-by-step solution
Where the answer comes from: the passage shows that beak depth is inherited and that the average shifted toward deeper beaks (9.4 mm → 9.8 mm) when hard seeds were the main food. The question now gives a new environment in which small, soft seeds dominate and shallow beaks handle them faster.
Step by step:
The population still contains variation in beak depth.
In the new conditions, shallow-beaked finches get food more efficiently, so they are likely to survive and raise more young.
Their offspring inherit shallower beaks, so over several generations the average beak depth would decrease.
Answer: The average beak depth would likely decrease, because finches with shallower beaks would survive and reproduce more.
Check: this kind of back-and-forth has actually been recorded in island finches: average beak size rises in dry years and falls again in wet years.
Tip: natural selection has no goal or fixed direction. When the environment changes, the favored trait can change too.
Question 30 of 36
Whales With Legs
Whales live their whole lives in water, but they are mammals: they breathe air, are warm-blooded and nurse their young. Paleontologists hypothesize that whales evolved from four-legged mammals that lived on land. The table summarizes several fossil animals and modern whales. Ages are approximate.
Animal
Age (millions of years ago)
Description
Pakicetus
50
Four legs; lived on land near water; ear bones shaped like those of modern whales
Ambulocetus
49
Four short legs and large feet; lived both on land and in water
Rodhocetus
47
Short hind legs; probably swam using its tail and legs
Basilosaurus
40
Lived only in water; tiny hind legs that could not support its body
Modern whales
Today
No hind legs; small pelvic and leg bones inside the body, not attached to the spine
Which discovery, if confirmed, would most weaken the hypothesis that whales evolved from four-legged land mammals?
Hint
The hypothesis predicts an order in time: legged ancestors first, legless whales later. Which finding would break that order?
Step-by-step solution
Where the answer comes from: the table shows an order in time: Pakicetus (50 million years ago, four legs) → Ambulocetus (49) → Rodhocetus (47, short hind legs) → Basilosaurus (40, tiny hind legs) → modern whales (no hind legs).
Step by step:
If whales came from four-legged land mammals, fully aquatic legless whales should appear after their legged ancestors.
A fully aquatic whale with no hind legs in rocks 70 million years old would be 20 million years older than Pakicetus. That would put the "end result" before the supposed ancestors and break the predicted order.
The other choices fit the hypothesis: a 45-million-year-old fossil with medium-sized hind legs fills a gap in the series; land-mammal-like teeth and hair in whale calves both link whales to land mammals.
Answer: A fossil of a fully aquatic whale with no hind legs, found in rock about 70 million years old.
Check: scientists would first re-check the dating, because one finding that conflicts with many others needs strong confirmation, which is why the question says "if confirmed."
Tip: to weaken a hypothesis, look for evidence that the hypothesis says should not exist, such as a descendant appearing before its ancestors.
Question 31 of 36
Comparing Primate DNA
Scientists compared long stretches of DNA from humans and several other primates. The table shows the approximate percent of DNA bases that differ between humans and each species.
After two groups split from a common ancestor, small changes build up in each group's DNA slowly and fairly steadily. So fewer differences suggest a more recent common ancestor.
Species
DNA difference from humans (%)
Chimpanzee
1.2
Gorilla
1.6
Orangutan
3.1
Rhesus macaque (a monkey)
7.0
A student uses only the data in the table to draw a cladogram of the five primates (human, chimpanzee, gorilla, orangutan and rhesus macaque). Which feature should the cladogram have?
Hint
The species with the most DNA differences from humans split from the human line earliest, so it branches off lowest on the cladogram.
Step-by-step solution
Where the answer comes from: the passage says "fewer differences suggest a more recent common ancestor." The table orders the species from fewest to most differences: chimpanzee 1.2, gorilla 1.6, orangutan 3.1, macaque 7.0.
Step by step:
The macaque (7.0%) shares the oldest common ancestor with humans, so its branch splits off first, at the bottom.
Next comes the orangutan (3.1%), then the gorilla (1.6%).
The chimpanzee (1.2%) splits off last, so it shares the highest node with humans and sits next to the human branch.
Answer: The macaque's branch splits off first (lowest), and the chimpanzee's branch splits off last, closest to the human branch.
Check: this matches what many other studies have found: chimpanzees and humans are each other's closest living relatives, with gorillas next and orangutans further away.
Tip: to turn a "differences" table into a cladogram, put the most different species at the bottom and the least different species next to the reference species at the top.
Question 32 of 36
Comparing Primate DNA
Scientists compared long stretches of DNA from humans and several other primates. The table shows the approximate percent of DNA bases that differ between humans and each species.
After two groups split from a common ancestor, small changes build up in each group's DNA slowly and fairly steadily. So fewer differences suggest a more recent common ancestor.
Species
DNA difference from humans (%)
Chimpanzee
1.2
Gorilla
1.6
Orangutan
3.1
Rhesus macaque (a monkey)
7.0
A museum visitor says, "If humans evolved from chimpanzees, why are there still chimpanzees?" Based on the passage, which response best corrects the visitor's misunderstanding?
Hint
Re-read the passage: what does a small DNA difference suggest the two species share?
Step-by-step solution
Where the answer comes from: the passage says that after "two groups split from a common ancestor, small changes build up in each group's DNA," so "fewer differences suggest a more recent common ancestor." The chimpanzee's 1.2% difference is the smallest in the table.
Step by step:
The visitor assumes humans came from chimpanzees. The data say something different: humans and chimpanzees share a common ancestor.
After the split, both lineages kept changing. Modern chimpanzees are as "evolved" as modern humans; they are cousins, not ancestors.
So there is no puzzle: the common ancestor's population split into two groups, and each group gave rise to a different living species.
Answer: Humans did not evolve from chimpanzees; humans and chimpanzees both descended from a common ancestor, and each line changed separately after they split.
Check: a 1.2% difference across billions of DNA bases still means millions of differences, so the two are clearly separate species.
Tip: living species are never the ancestors of other living species. They are branch tips that meet at shared ancestors further back.
Question 33 of 36
Lizards on Two Islands
Scientists are studying two populations of a small lizard that live on neighboring islands, Island A and Island B. Thousands of years ago, the islands were joined by a strip of land, and the lizards formed a single population. Then the sea level rose and covered the land bridge. The lizards cannot swim between the islands, so the two populations no longer mix.
Over many generations, the populations have changed in different ways. Island A is rocky, with few plants, and its lizards now have longer legs on average, which help them run quickly across open rock. Island B is covered with thin bushes, and its lizards have shorter legs on average, which help them grip narrow branches. Genetic tests show that the two populations descended from the same ancestral population.
The scientists want to know whether the two populations have now become separate species.
Which additional finding would provide the strongest evidence that the lizards on Island A and Island B have become separate species?
Hint
Recall the biological definition of a species. Which finding is about the ability to interbreed, not just about looks or habits?
Step-by-step solution
Where the answer comes from: the passage describes physical differences (leg length) and shared ancestry ("descended from the same ancestral population"), and it ends: "The scientists want to know whether the two populations have now become separate species."
Step by step:
By the biological species concept, a species is a group whose members can interbreed and produce fertile offspring. Separate species are reproductively isolated.
So the strongest evidence is a direct test of reproduction: when lizards from the two islands are brought together, they produce no offspring, or only offspring that cannot reproduce.
Check the others:
A difference in leg length is already known. Populations of one species can look different (consider the many breeds of dogs, which are all one species).
A difference in diet is a behavioral difference that does not show the populations cannot interbreed.
Shared ancestry is expected for any two populations that split from one, whether or not they have become separate species. It does not answer the question.
Answer: When lizards from the two islands are placed together, they produce no offspring, or only offspring that cannot reproduce.
Tip: for "strongest evidence" questions, pick the choice that tests the definition directly. Looks, habits and shared history are weaker clues than reproductive isolation.
Question 34 of 36
Two populations of tree frogs live in the same ponds. Frogs in Population 1 breed in early spring. Frogs in Population 2 breed in late spring, about six weeks later, so in nature the two populations almost never mate with each other. When scientists bring males and females from the two populations together in a lab, they can still mate and produce healthy offspring.
Which statement best describes what could happen to these populations over many generations?
Hint
The populations share the same ponds, so there is no physical barrier. What else can keep their genes from mixing?
Step-by-step solution
Where the answer comes from: the stem says the two populations breed six weeks apart, "so in nature the two populations almost never mate with each other," although they still can in a lab.
Step by step:
New species form when populations stop exchanging genes (reproductive isolation). The barrier can be geographic, but it can also be timing, behavior or habitat.
Here the barrier is time: different breeding seasons keep the two gene pools apart even in the same ponds.
With no gene flow, mutations, natural selection and chance can make the populations more and more different. Eventually they might no longer be able to interbreed at all; then they would be separate species.
Right now they can still produce healthy offspring, so they are not yet fully separate species.
Answer: Because they breed at different times, their genes rarely mix, so differences could build up until they become separate species.
Check: this is the same logic as island populations separated by water, except that the separation here is in time rather than space.
Tip: for speciation questions, ask "What stops the two groups from interbreeding?" Any barrier that stops gene flow, not only mountains or water, can start speciation.
Question 35 of 36
Antibiotic Resistance in a Hospital
Staphylococcus aureus is a common bacterium that can cause skin and blood infections. In 2000, doctors at a large hospital began using a new antibiotic widely. Each year, the hospital lab tested samples of the bacterium taken from patients to find what percent could survive the antibiotic (were resistant).
Even before 2000, a few bacteria in the population carried a gene variant that let them survive the antibiotic. Resistance is inherited: when a resistant bacterium divides, both new cells are resistant. In 2014, the hospital started a program that cut the use of the antibiotic roughly in half.
A hospital official claims: "Our 2014 program caused the rise in antibiotic resistance to slow down." Which statement best evaluates this claim?
Hint
Did resistance keep rising after 2014, and how fast? Then ask: does the study include anything to compare with, such as a hospital without the program?
Step-by-step solution
Where the answer comes from: the graph shows increases of 16 points (2008–2012), 8 points (2012–2016) and 2 points (2016–2020). The passage says the program began in 2014 and "cut the use of the antibiotic roughly in half."
Step by step:
Resistance kept rising after 2014 (38 → 46 → 48), but much more slowly. So the data are consistent with the claim.
Less antibiotic use means weaker selection for resistant bacteria, so a slowdown makes biological sense.
But the graph alone cannot prove cause: the 2012–2016 interval includes two years before the program began, so the graph cannot show exactly when the slowdown started, and there is no comparison hospital without the program. Other factors (for example, better hand-washing) could also explain the change.
Answer: The data are consistent with the claim, because resistance rose more slowly after 2014, but they cannot prove the program caused the slowdown, because there is no comparison group without the program.
Check: "resistance kept rising" does not contradict the claim, which is about the rate of rise, not about resistance going down.
Tip: a change that follows an action in time is evidence that fits a causal claim, not proof of it. Proof needs a controlled comparison.
Question 36 of 36
The Mexican tetra is a small fish. Populations that live in rivers have working eyes and silver-gray skin. Several populations that live in completely dark caves have no working eyes and almost no skin color. Genetic studies show that the cave populations descended from river fish that entered the caves long ago.
A student claims: "Evolution always makes organisms more complex by adding new structures." Which statement best evaluates the student's claim?
Hint
Did the cave populations gain new structures or lose existing ones? Does that fit the word "always"?
Step-by-step solution
Where the answer comes from: the stem says river populations "have working eyes and silver-gray skin," cave populations "have no working eyes and almost no skin color," and the cave fish "descended from river fish."
Step by step:
The cave fish evolved from ancestors that had eyes and color, and they lost both features.
That is evolution (an inherited change in a population over generations) that made the fish simpler, not more complex.
One clear counter-example is enough to disprove a claim that uses the word always.
In total darkness, eyes give no advantage and cost energy to build, so fish without them may do just as well or better. Evolution favors what works in the environment, not what is more complex.
Answer: The cave fish contradict the claim, because these populations evolved by losing eyes and skin color, becoming less complex.
Check: the change is inherited (shown by the genetic studies), so it counts as evolution, not just a change in individual fish.
Tip: evolution is not a march toward complexity. Parasites, cave animals and flightless island birds have all lost structures that their ancestors had.